![Lab Experiments](https://static.wixstatic.com/media/11062b_b8f54a47fba14e8d82bb5a334f95b0bc~mv2.jpg/v1/fill/w_27,h_18,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/11062b_b8f54a47fba14e8d82bb5a334f95b0bc~mv2.jpg)
Liquid Culture
Liquid culture is a nutritious solution that mycelium can grow on. You can use it to store your species, create your own syringes and have a free long-term supply.
Key benefits of liquid culture:
-
Quicker grain colonisation time
-
Longer shelf life
-
Lower contamination risk
-
Scalability - can create larger quantities
-
More uniform and vigorous growth
-
Reduces the need to buy spores syringes from vendors
You are 100% capable of creating your own liquid culture, give it a go today!
How To Create Liquid Culture
Equipment:
-
Pre-made Liquid Culture Pouch (Our 100g pouch can create 25L !)
-
Liquid Culture Jars
-
Syringes + Needles
-
Magnetic Stirrer
-
Stir Bars
Our liquid culture kit contains all the above, view HERE:
Additional Extras
-
Pressure Cooker (we recommend the Hawkins brand available on Amazon)
Best Liquid Culture Recipe
1. Prepare Nutrient Solution
Mix 4g of Liquid Culture Pre-Mix per litre of distilled water.
Stir thoroughly to dissolve the malt extract completely.
2. Sterilise the Nutrient Solution
Pour the nutrient solution into your liquid culture jar, and add a stir bar. You can purchase a pre-made liquid culture jar HERE.
Seal the container with a lid, and then place it in a pressure cooker.
Sterilize the nutrient solution at 15 psi for 15 minutes.
3. Cooling and Inoculation
Allow the sterilized nutrient solution to cool to room temperature before proceeding.
Using a sterilized needle, inoculate the liquid culture with a spore or mycelium solution.
4. Incubation
Place the inoculated liquid culture in a warm, dark area for incubation (24-27°C).
5. Monitor and Spin
Regularly inspect the liquid culture for signs of mycelial growth.
You can use a magnetic stirrer to distribute the mycelium throughout the solution or shake the container gently.
Mycelium growth may take several days to a few weeks, depending on the species and conditions.
6. Harvesting and Use
Once the liquid culture is fully colonised with mycelium, it is ready for use. You can use a magnetic stirrer to mix the mycelium before creating your syringe
Best Liquid Culture Recipe
When stored in a refrigerator at around 4°C (39°F), liquid cultures can last for 6 months to a year refrigerated as it slows down the metabolic activity of the mycelium.
Ready to make your own liquid culture?
Which is better, spore syringe or liquid culture? Find out Here
![Laboratory Borosilicate Glass Reagent Bottle (19).png](https://static.wixstatic.com/media/4970d5_7469bb97cd364b6481d8602a8bd502d4~mv2.png/v1/crop/x_357,y_454,w_1889,h_2059/fill/w_290,h_316,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/Laboratory%20Borosilicate%20Glass%20Reagent%20Bottle%20(19).png)
Spore Syringe Vs Liquid Culture Article
Liquid Culture Tutorial
![](https://static.wixstatic.com/media/4970d5_9d24a48a074648c1bd80568c4aa1f729f000.jpg/v1/fill/w_90,h_160,al_c,q_80,usm_0.66_1.00_0.01,blur_2,enc_auto/4970d5_9d24a48a074648c1bd80568c4aa1f729f000.jpg)