Liquid Culture

Liquid culture is a nutritious solution that mycelium can grow on.  You can use it to store your species, create your own syringes and have a free long-term supply.

 

Key benefits of liquid culture: 

• Quicker grain colonisation time

• Longer shelf life

• Lower contamination risk

• Scalability - can create larger quantities

• More uniform and vigorous growth

• Reduces the need to buy spores syringes from vendors

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How To Create Liquid Culture

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Equipment:

• Pre-made Liquid Culture Pouch (Our 100g pouch can create 25L !)

• Liquid Culture Jars

• Syringes + Needles

• Magnetic Stirrer 

• Stir Bars 

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Our liquid culture kit contains all the above, view HERE: 

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Additional Extras

• Pressure Cooker (we recommend the Hawkins brand available on Amazon)

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Best Liquid Culture Recipe

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1. Prepare Nutrient Solution

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Mix 4g of Liquid Culture Pre-Mix per litre of distilled water.

Stir thoroughly to dissolve the malt extract completely.

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2. Sterilise the Nutrient Solution

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Pour the nutrient solution into your liquid culture jar, and add a stir bar. You can purchase a pre-made liquid culture jar HERE. 

Seal the container with a lid, and then place it in a pressure cooker.

Sterilize the nutrient solution at 15 psi for 15 minutes.

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3. Cooling and Inoculation

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Allow the sterilized nutrient solution to cool to room temperature before proceeding.

Using a sterilized needle, inoculate the liquid culture with a spore or mycelium solution. 

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4. Incubation

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Place the inoculated liquid culture in a warm, dark area for incubation (24-27°C).

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5. Monitor and Spin

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Regularly inspect the liquid culture for signs of mycelial growth. 

You can use a magnetic stirrer to distribute the mycelium throughout the solution or shake the container gently. 

Mycelium growth may take several days to a few weeks, depending on the species and conditions.

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6. Harvesting and Use

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Once the liquid culture is fully colonised with mycelium, it is ready for use. You can use a magnetic stirrer to mix the mycelium before creating your syringe

 

 

Best Liquid Culture Recipe

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When stored in a refrigerator at around 4°C (39°F), liquid cultures can last for 6 months to a year refrigerated as it slows down the metabolic activity of the mycelium.

 

Ready to make your own liquid culture?

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Which is better, spore syringe or liquid culture? Find out Here