This kit contains everything you need to make your own liquid culture! 
 
Includes:
2 x 1000ml Liquid Culture Jars (empty)
4 x 10ml Syringes + Needles
1  x Liquid Culture Pre-mix Pouch 100g
 
Optional extras:
1 x Magnetic Stirrer
2 x Magnetic  Stir Bars
 
Instructions for Use:
 
1. Prepare Nutrient Solution:
Mix 4g of Liquid Culture Pre-Mix per litre of distilled water.
Stir thoroughly to dissolve the malt extract completely.
 
2. Sterilise the Nutrient Solution:
Pour the nutrient solution into your liquid culture jar, and add a stir bar. You can purchase a pre-made liquid culture jar HERE. 
Seal the container with a lid, and then place it in a pressure cooker.
Sterilize the nutrient solution at 15 psi for 15 minutes.
 
3. Cooling and Inoculation:
Allow the sterilized nutrient solution to cool to room temperature before proceeding.
Using a sterilized needle, inoculate the liquid culture with a spore or mycelium solution. 
 
4. Incubation:
Place the inoculated liquid culture in a warm, dark area for incubation (24-27°C).
 
5. Monitor and Spin:
Regularly inspect the liquid culture for signs of mycelial growth. 
You can use a magnetic stirrer to distribute the mycelium throughout the solution or shake the container gently. 
Mycelium growth may take several days to a few weeks, depending on the species and conditions.
 
6. Harvesting and Use:
Once the liquid culture is fully colonised with mycelium, it is ready for use. You can use a magnetic stirrer to mix the mycelium before creating your syringe
 
Storage: 
When stored in a refrigerator at around 4°C (39°F), liquid cultures can last for 6 months to a year refrigerated as it slows down the metabolic activity of the mycelium.